Understanding Progesterone Testing & Determining Optimal ... Please follow this chart and give to your veterinarian when planning for a breeding. We know the progesterone level at the time of ovulation. Progesterone levels are usually less than 1.0 ng/ml before the LH spike, rising to 3 to 4 ng/ml the day following the LH spike. When is an elective C-section safe? Progesterone Testing - My Budget Vet The most fertile time to breed is 2 to 4 days after ovulation (C4). Vaginal … read more. Using our lab o vulation occurs at about 3.8-5.0 ng/ml (11.7 nmol/L) of progesterone (including the day of ovulation and the 24 hours it takes to ovulate) and the best days to breed occur at about 17.6-24.1 ng/ml (54-74 nmol/L) of progesterone. Typically we like to pull progesterone levels on Day 5 of the bitches heat cycle. The Reproductive Dog Breeding Cycle Can Be Tricky! Estrus: Is the period of receptivity. Progesterone timing at the end of pregnancy. Once a bitch hits 5ng and ovulates, subsequent serum progesterone levels can vary greatly. The duration of pregnancy has always been regarded as 63-65 days from the first mating. However, at the time of breeding, there is no "ideal" level. Dogs, however, ovulate when the oestrogen level is declining and the progesterone level is increasing. Variability in the timing of ovulation, length of oestrus and multiple breeding dates can make it difficult to predict an exact due date for a litter. Christine Ovulation occurs when progesterone blood levels reach 5-6ng/mL. This type of ovulation timing is used for many reasons…fresh chilled and frozen breeding, infertility evaluations, natural breeding with limited stud access, and breedings with older males or dogs with poor semen quality. This is most effective when you can get progesterone results back the same day the sample is drawn and sent to the lab. However, at the time of breeding, there is no "ideal" level. Progesterone levels are usually less than 1.0 ng/ml before the LH surge, rising to 3 to 5 ng/ml the day following the LH surge. Progesterone, another important reproductive hormone in the bitch, is present in low levels before the LH surge and then begins to rise around the time of the LH surge. The dramatic change in Progesterone levels during the breeding period renders it an excellent tool for ovulation timing. 5 part series all about progesterone levels in breeding dogs All about dog breeding www.LoveMyPups.comDon't forget, subscribe, like and make comments, Our st. Larger dogs tend to reach puberty at an older age than smaller dogs. So you need certain assurance that your bitch will be 100% ready to breed at a specific time. These areas on the ovaries are where progesterone is produced in levels high enough to maintain In order to interpret results one needs to be aware of the unique pattern of progesterone secretion in the bitch. A progesterone below 3 ng/dl indicates a c-section is safe, as long as the dog does not have luteal failure. Ovulation happens when progesterone blood levels reach 5 or 6ng/mL. We determine breeding days for natural or transcervical breeding at 3 and 5 days after the . Thereafter the progesterone is > 5 ng/ml. Normally present in low doses until ovulation occurs, levels of progesterone start to climb around the time of the LH (luteinizing hormone) peak. The fertile period of breeding is generally recommended as the 3rd to the 5th days of estrus, or every . You can usually wait to do the first progesterone number on the 8th day. Progesterone is best used in conjunction with other monitoring techniques, such as observation of clinical signs, vaginal cytology, vaginoscopy, ultrasound, and additional hormonal testing. The breeding protocol should be determined based on the estimated day of ovulation. Broadly: Progesterone is over 6.5 nmol/L (2 ng/ml) at the LH peak: breed 4-6 days later. Canine Birth and C-Section Timing Keep the danger at bay. Call the stud owner as soon as the bitch is showing signs . Thus, a bitch should begin breeding 2-4 days after the progesterone rises over 2 ng/ml. Both vets had recommended breeding on the 24th and 25th based on levels and even on the 23rd when the result of 11 was given they told her to breed the next day. Progesterone levels. Progesterone levels are not species specific, which means that the test can be run in almost any veterinary lab, or in even human labs. progesterone level. The ovulation date can be as early as day 7 and as late as day 27 of the estrous cycle, emphasizing the importance of the blood test. 2.0 to 5.0: Test every day. The reason it is so difficult to time ovulation in the absence of hormone testing is that the stages of the canine estrous cycle vary considerably in length. When fertility is optimal, progesterone values might be around 10, 30, sometimes even greater than 60ng/mL. Timing of breeding The aim is to identify when the progesterone level reaches 2.5 ng/ml so the mating schedule can be set up, or the veterinarian and owner of the male dog can be notified that they should be prepared to collect and ship a semen sample or to pinpoint the optimum time for breeding A drop in progesterone to < 2 ng/mL (from C4 to dark C2 or C1) indicates whelping will occur within 24 - 36 hours. These areas on the ovaries are where progesterone is produced in levels high enough to maintain This value is the same whatever the size of the dog. The average price for taking a blood sample, performing the progesterone test and having a vet look over the results is $45 or £50. When the progesterone level rises to 5 ng/ml, ovulation occurs. Whether you have a small or large breed, your bitch should ovulate at around the same progesterone level as any other dog. General Timing for Natural Breeding - Progesterone units here are ng/mL so you look for: 2 to 2.9 breed in 4 days & 3 to 3.9 breed in 3 days & 4 to 8 breed today or tomorrow & 8 to 15 breed immediately. Ovulation occurs at a progesterone level of 5 ng/ml. After ovulation, progesterone concentrations continue to increase for 2-3 weeks reaching as much as 80 ng/ml, this level is necessary to maintain the pregnancy. What level of progesterone indicates ovulation in dogs? The units they deliver the results in are normally ng/ml (nanogram per millilitre of blood). The surgery to implant the semen is a Dogs, however, ovulate when the estrogen level is declining and the progesterone level is increasing. Serum Progesterone for Estimating Day of Parturition in the Bitch. The breeding protocol should. 22,572 satisfied customers. Using our lab ovulation occurs at about 3.8-5.0 ng/ml (11.7 nmol/L) of progesterone (including the day of ovulation and the 24 hours it takes to ovulate) and the best days to breed occur at about 17.6-24.1 ng/ml (54-74 nmol/L) of progesterone. August 21st 4.7. Even spayed bitches run basal levels of progesterone. If you get readings that do not make sense, then re-test. Occasionally, medical intervention will suffice, including calcium or magnesium supplementation. Progesterone testing is used to determine when a bitch ovulates and thus when to breed. Progesterone can also be used to confirm that ovulation did take place- levels over 15.0 ng/ml can be used retrospectively to confirm ovulation, but does not help in future planning of a breeding of that cycle. Experienced dog breeders are aware of the value of progesterone testing. As the cycle continues, progesterone levels slowly climb to a level of 5ng, upon which ovulation occurs. Determine breeding schedule based on desired method of insemination and semen type. However, this relationship is not well established and there is considerable variation in serum progesterone concentrations after the initial rise. Dogs, however, ovulate when the estrogen level is already declining and the progesterone level is increasing. Blood progesterone levels and fetal monitoring (by ultrasound) are most critical. August 24th 23. Progesterone level monitoring is most effective and easy to use. Progesterone is at baseline concentrations (<1 ng/ml). It is easy to test for and can be used to look at ovulation, parturition and other cyclical abnormalities. "It is better to know the quantitative progesterone level (measured in nanograms), which can be determined by a commercial laboratory. Behaviorally it begins the first day the bitch stands. Progesterone can also be used to estimate the date of whelping. When testing progesterones at the end of pregnancy, we frequently see . Results should also be available in less than 24 hours, which is important when working in a tight timeframe for breeding. Thus if a progesterone result is above 6 nmol/L in a sample collected a month or more after exposure to a male, the most likely reason for the result is pregnancy. This method of deciding when to mate should improve our pregnancy rates from around 70% to 95%. The overall investment including also the cost of a quality bitch will be a lot of money. When fertility is optimal, progesterone values might be around 10, 30, sometimes even greater than 60ng/mL. If you get readings that do not make sense, then re-test. When the progesterone level is between "4" (55)- "5" (65). Also is useful in determining whelping dates, allowing the owner prepare for the c-section (cesarean) weeks in advance. A progesterone below 3 ng/dl indicates a c-section is safe, as long as the dog does not have luteal failure. 1. Ovulation will occur anytime when progesterone is between 5ng/ml and 10ng/ml. Breeding with fresh semen, by either natural breeding or vaginal AI with fresh semen, can be done on the day of ovulation, when the progesterone level reaches 5 ng/ml. Progesterone levels can sometime double in 24 hours so daily testing is imperative. On a side note-this elevated progesterone level is what causes the symptoms of a false pregnancy when a bitch is not pregnant. Progesterone Cycle in the Bitch Be secure. Breeding with fresh semen, by either natural breeding or vaginal AI with fresh semen, can be done on the day of ovulation, when the progesterone level reaches 5 ng/ml. When fertility is optimal, progesterone values might be around 10, 30, sometimes even greater than 60ng/mL. When the progesterone level rises to 5 ng/ml, ovulation MAY . It is recognized that in the dog up to 75% of failures to conceive can be attributed to incorrect timing of breeding. 15-22: Your bitch is ready to . LH causes the luteinization of the ovarian follicles after the eggs are released. It can vary from one cycle (or heat) every 18 months (this is typical of some of the giant breeds) to one every 4 months. We would like to minimize misses based on timing. If the reading is 9, you're too late for a natural breeding, if 0.9, you're too early. The dog progesterone test kit is specific to the state-of-the-art Cube Vet analyzer shown here. However, for the best results, many breeders will mate their dogs five and six days after the bitch's luteinizing hormone surge. When the progesterone level rises to 5 ng/ml, ovulation occurs. Canine progesterone blood testing takes away all the most important uncertainty out of dog breeding. This indicates that ovulation is imminent. Progesterone, another important reproductive hormone in the bitch, is present in low levels before the LH surge and then begins to rise around the time of the LH surge. This baseline progesterone is probably of adrenal origin. It improves breeding efficiency and provides confidence for the breeders and veterinarians that the dog and bitch are mating on the correct day. The progesterone levels, like the speed of the car, is determined by the individual. August 23rd 11. Many breedings are done far too early and result in misses. conducted a comparative study of three different methods of measuring the level of progesterone in blood samples from bitches. A canine's progesterone levels can be tested every two to three days starting about . Progesterone is at baseline concentrations (<1 ng/ml). A reproductive hormone, progesterone increases in the bloodstream just before ovulation. Progesterone levels should be tested every two to three days once the dog is five(5) to seven(7) days into heat. This represents a huge saving in lost time of empty bitches and generally maximising litter size as well. Although the eggs are not mature yet, most fresh semen is viable enough to survive in the bitch's reproductive tract until the eggs are ready. Progesterone is over 16 nmol/L (5 ng/ml) at ovulation. It is important to know that if the rise in progesterone is missed, guesswork is needed to determine the actual day for the breeding. As a result, the prime time to breed is 48 to 72 hours post ovulation." Progesterone kits that chart progesterone levels through color changes are available to breeders from veterinarians. dog progesterone level chart dog ovulation cycle. The fertile period of breeding is generally recommended as the 3rd to the 5th days of estrus, or every . Larger dogs tend to reach puberty at an older age than smaller dogs. Breeding the bitch on days 3 and 5 (or 4 and 6) after the LH surge ensures the maximum conception rate and largest litter sizes. Progesterone is best used in conjunction with other monitoring techniques, such as observation of clinical signs, vaginal cytology, vaginoscopy, ultrasound, and additional hormonal testing. Interpretation of Serum Progesterone Results for Management of Breeding in Dogs We usually start natural breeding's the day the progesterone goes over 10. The perfect bitch will ovulate when her progesterone levels reach 5ng/ml. Why Progesterone Testing Is Important for Dog Breeding August 14, 2018 10:58 pm Published by Writer Leave your thoughts. . August 22nd 5.1. To time C-section or whelping, test progesterone starting 58 - 60 days after first breeding (average gestation 63 days). Progesterone levels for the bitch: Monday=2.5, Tuesday=2.3. Progesterone is the most important hormone in female dog reproduction. My name is ***** ***** and I am trying to breed two Labs. . The perfect bitch will ovulate when her progesterone levels reach 5ng/ml. The breeding protocol should be determined based on the estimated day of ovulation. Whether you have Chihuahuas, Labrador Retrievers, Mastiffs, etc., bitches will always ovulate around the same progesterone level. LH tests must be done daily and the blood must be drawn at approximately the same time each day. When testing progesterones at the end of pregnancy, we frequently see . Eggs are released (ovulated) 44 hours (2 days) after the LH surge, on average. Progesterone Levels Predict Breeding Results. Assessment of different methods of measuring progesterone. Large and small animal Vet. Over 8.0 ng/ml with no white sells - then the bitch has ovulated When you hit the level above 4.0 ng/mL this is day 1 of ovulation. Be safe. Some progesterone levels advance really quick, like the car off the line in a 1/4 mile test and . Here are the progesterone levels. Progesterone levels of 35.0 - 40.0 ng/ml on the miniVidas results; LH testing is rarely used today, however, it was most used when planning a frozen semen breeding or when breeding a bitch that has a history of difficulty of conceiving. The timing of the breeding, based on progesterone levels (bred 2 to 3 days post-ovulation depending on semen type used), was just right. Blood samples will be taken every 2-3 days until the serum progesterone level reaches 4-10, this is the day of ovulation. Progesterone levels are usually less than 1.0 ng/ml before the LH spike, rising to 3 to 4 ng/ml the day following the LH spike. Serial progesterone measurements can be collected by sampling every 1 to 4 days - increasing in frequency as the sharp rise in progesterone approaches. Progesterone: Maintains a pregnancy. Ovulation will occur anytime when progesterone is between 5ng/ml and 10ng/ml. eminent to prevent delivery of premature puppies. What we are looking for is a progesterone level of 20nmol/l. With one easy, on-site test, detect progesterone to accurately predict ovulation and target optimal breeding dates […] This is most effective when you can get progesterone results back the same day the sample is drawn and sent to the lab. In the dog, the progesterone level will remain high for about 60 days whether or not the dog is bred, and whether or not she is pregnant. Most mammals ovulate when the estrogen level in the blood is increasing. Most breeders will opt for a live AI instead of letting the dogs mate in order to avoid injury to the stud dog. Doctoral Degree. Progesterone levels and luteinizing hormone (LH) levels are the best indicators of when ovulation will take place and when is the best time to breed. Most veterinarians recommend breeding 2 days after ovulation with fresh and fresh chilled shipped semen and 3 days with frozen semen. August 17th 1.4. If doing a natural breeding, I generally breed the bitch the day of ovulation or the day after, and then two days later(2-3 days after ovulation). Once you start to see the level at: 2.0 to 2.9 ng/ml = breed in 4 to 5 days 3.0 to 3.9 ng/ml = breed in 3 to 4 days 4.0 to 8 .0 ng/ml = breed today or tomorrow - this is where the LH Surge Occurs. If previous breeding history is unknown, begin progesterone testing 4-6 days after the onset of heat. . We are proud to be a Brucellosis-free hospital; We require a Brucellosis negative certificate within the last 6 months for all breeding dogs; Same day results along with a certificate of the negative test status; Breeding Timing with Progesterone Levels We cannot say that the bitch should be bred when she is at [any number] ng/mL of progesterone. However, some bitches who haven't read the book and can vary considerably. Estrogen levels can give us a general idea of when a dog will come into "standing" heat, but are not sufficient to determine when breeding should actually take place for optimal conception rates. It's also one that requires you to be aware of a variety of details about your dog's health and wellness. When it has been determined that the dog has ovulated, peak fertility is 48 hours after a progesterone result of 4-10 is noted. Idexx also recommend when it is you go to mate the dog or if another test is needed (usually 2 days later if the progesterone level was too low for an accurate prediction of ovulation). If your female's progesterone reading is 32-64 nmol/L or 10.06-20.13 ng/mL the eggs have matured and mating should occur within 2 days for optimal fertility. In 2010, Moxon et al. In my last breeding, the dog had three tests with progesterone readings of 3+ and then a reading of 5. Accurate breeding history, especially LH peak, is also very valuable. The rise from a 3 to 7 progesterone level is believed to . 6. Explore further detail here. Important note: The above are guidelines, and individual dogs may vary. The breeding protocol should be determined based on the estimated day of ovulation. We are in the process of breeding our French Bulldog. Progesterone Levels Predict Breeding Results Progesterone levels should be tested every two to three days once the dog is three to five days into heat. We cannot say that the bitch should be bred when she is at [any number] ng/mL of progesterone. The eggs are viable for about 3 - 4 days after this (5 - 6 days after the LH surge). Dog breeding is a complicated business. The ideal progesterone level for mating within 1-3 days is 19-31 nmol/L or 5.97-9.75 ng/mL, readings within this range indicate that ovulation has recently occurred. The hormone progesterone (P4) is an androgen produced by the corpus luteum of bitches. However, most commonly, surgery is required. There are some reports that a serum progesterone concentration of 4-10 ng/ml occurs in association with ovulation. For fresh chilled and frozen semen, we usually want the progesterone between 15 and 22. Determine breeding schedule based on desired method of insemination and semen type. Most bitches ovulate between a 3ng and 7ng so watch for a 5.0 as your benchmark. Your dog could be ready almost any day. The Guide Dogs Breeding Centre takes blood samples from their bitches as part of their reproductive management, so it was a valuable . 1. August 20th 1.9. Progesterone timing at the end of pregnancy. Number Results Quantitative Number Results in Minutes Breed with confidence. The first vaginal artificial insemination should be performed 48 hours after the progesterone reaches 5 ng/ml. Even spayed bitches run basal levels of progesterone. Monitor your dog's progesterone levels to determine the proper C-section timing. . Her progesterone levels should also rise from basal levels to over 5 ng/ml. Brucellosis Test. Hormonally it begins on the day of the LH surge and is marked by rising progesterone levels and decreasing estrogen levels. At what progesterone level does a dog ovulate? This is the cost per test and many breeders will take 2 or even 3 tests over several days until they get the correct date and progesterone levels that signal the dog is ready to breed. You want healthy puppies! LIVE BREEDING TIMING Venomline's Mrs. Dolce 24 hours (1 day) after progesterone. Most professional dog breeders use either natural or artificial insemination methods to maximize the success of the breeding. Continued sampling will confirm ovulation. At what progesterone level should I breed my dog? Understanding how the hormone levels change can help in determining the best time to breed and when to anticipate whelping. Progesterone testing is usually done every other day and, in the early days of the cycle, may be done every 3 to 4 days. The dog was bred using side by side. We then recommend mating that day and in 48 hours. As the progesterone level increases, lutenizing hormone (LH) is released from the pituitary gland, triggering the release of eggs from the follicles. LH causes the luteinization of the ovarian follicles after the eggs are released. The average bitch will cycle approximately every 6 months and an individual bitch will tend to conform to her own unique repeating pattern throughout her fertile years. The progesterone test (needle of the speedometer) reflects the amount of progesterone being released. If the level is less than 5ng /ml another value should be checked a few days later. Dr. Loretta. The first vaginal artificial insemination should be performed 48 hours after the progesterone reaches 5 ng/ml. With my dogs the progesterone spike seems to happen 9-11 days following first color, but that varies from dog to dog. This baseline progesterone is probably of adrenal origin. Pre-Breeding Screening & Testing. If the levels of progesterone are baseline, then the dog should be retested every 3-4 days until a level of progesterone is detected that is consistent with the onset of the LH peak. Diestrus: Normally begins 7 to 9 days post the actual LH surge . If a breeder is not in a position to do a complete progesterone level series there is still an added value running a progesterone level when the bitch starts flagging and again on the first breeding day. Important note: The above are guidelines, and individual dogs may vary. When fertility is optimal, progesterone values might be around 10, 30, sometimes even greater than 60ng/mL. Please invest in progesterone numbers so you have the best chance to get a litter! levels preparing the reproductive tract for breeding. 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